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262 antibody  (R&D Systems)


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    R&D Systems 262 antibody
    262 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/262+antibody/10__1158_slash_1535___7163__mct___25___0136-138-33-37?v=R%26D+Systems
    Average 93 stars, based on 17 article reviews
    262 antibody - by Bioz Stars, 2026-06
    93/100 stars

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    The combined inhibition of three epigenetic silencing marks increases the fetal globin expression in HUDEP-2 cells. HUDEP-2 cells treated with lower doses of individual treatment (0.2 μM EML, 0.1 μM GSK, and 0.01 μM FTX) and together for a total of 9 days treatment (see ). ( A ) The percentage of fetal hemoglobin positive (HbF+) cells was determined by flow cytometry. Data represents mean ± SD from N=4 independent experiments. “Combination x3” indicates where all three inhibitors were used together. ( B ) Percentage of live cells quantified using a Bio-Rad cell counter and normalized to the DMSO control. Data represent mean ± SD from N=4 independent experiments. ( C, D ) Representative flow cytometry histogram plot showing HbF+ mean fluorescence intensity (MFI) (X-axis) vs. HbF+ cell count (Y-axis) with and without EML in HUDEP-2 cells, analyzed using FlowJo software. ( E ) Western blot analysis for indicated antibodies and ( F ) stain-free SDS-PAGE protein gel after cells were harvested at stage 3 for a total of 9 days of treatment. The two DMSO lanes as indicated by a bracket are the 2x dilution (1/2) of cell lysates.

    Journal: bioRxiv

    Article Title: Regulation of BCL11A DNA binding and expression in human erythrocyte precursor HUDEP-2 cells

    doi: 10.64898/2026.02.06.704516

    Figure Lengend Snippet: The combined inhibition of three epigenetic silencing marks increases the fetal globin expression in HUDEP-2 cells. HUDEP-2 cells treated with lower doses of individual treatment (0.2 μM EML, 0.1 μM GSK, and 0.01 μM FTX) and together for a total of 9 days treatment (see ). ( A ) The percentage of fetal hemoglobin positive (HbF+) cells was determined by flow cytometry. Data represents mean ± SD from N=4 independent experiments. “Combination x3” indicates where all three inhibitors were used together. ( B ) Percentage of live cells quantified using a Bio-Rad cell counter and normalized to the DMSO control. Data represent mean ± SD from N=4 independent experiments. ( C, D ) Representative flow cytometry histogram plot showing HbF+ mean fluorescence intensity (MFI) (X-axis) vs. HbF+ cell count (Y-axis) with and without EML in HUDEP-2 cells, analyzed using FlowJo software. ( E ) Western blot analysis for indicated antibodies and ( F ) stain-free SDS-PAGE protein gel after cells were harvested at stage 3 for a total of 9 days of treatment. The two DMSO lanes as indicated by a bracket are the 2x dilution (1/2) of cell lysates.

    Article Snippet: Cells were incubated with FITC-conjugated anti-human fetal hemoglobin antibody (REAfinity TM , clone REA533; Miltenyi Biotec #130-120-065) for 20 min at room temperature in the dark.

    Techniques: Inhibition, Expressing, Flow Cytometry, Control, Fluorescence, Cell Characterization, Software, Western Blot, Staining, SDS Page